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The Bible makes multiple references in Psalms to the use of music (including instruments) and singing in connection with worship. Jesus and the apostles were noted as having sung a hymn at the conclusion of the Last Supper (Matthew 26:30; Mark 14:26). Paul spoke in Ephesians 5:19 and Colossians 3:16 of Christians singing and making music in their hearts. James 5:13 mentions singing praise to God. Thus, the use of music and song in worship has a sound basis in Scripture. With specific reference to the Gregorian chant, this form of music (as best I can determine) did not have its origins or roots until the third century after Christ, and evolved during the following centuries. (Gregory I, for whom the form was named, was pope from 590 to 604.) In light of the significant time lapse between the close of the New Testament canon and the earliest roots of what would become known as Gregorian chant, I would say that the Bible (understandably) does not directly address this musical form. From the standpoint of general Christian principles, I would say that, as with any aspect of worship, the employment of such music would depend on the intent or underlying mindset of those who use it. Viewing or employing it simply as a matter of rote ritual performed out of habit or custom (with no serious thought being given to what is being said), or thinking of it as somehow having a special merit in God's eyes over other forms of worship or praise (regardless of the spiritual condition of the person offering it), would not be supported by Scripture.
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